Author(s):
1. Danijela Ćetković, Faculty of Natural Sciences and Mathematics, University of Tuzla , Republic of Srpska, Bosnia and Herzegovina
2. Oleg Lesmajster, Prirodno-matematički fakultet Banja Luka, Republic of Srpska, Bosnia and Herzegovina
3. Dragan Vuković, Republic of Srpska, Bosnia and Herzegovina
4. Bojana Milinković, Prirodno-matematički fakultet Banja Luka, Republic of Srpska, Bosnia and Herzegovina

Abstract:
Introduction: Caffeine is widely used alkaloid. It is a main ingredient of chocolatte, soft drinks, and paikillers and has strong effect on stimulaiton of central nervous system, cardiovascular function, gastovascular, respiratory and endocrine system. There is very little data about its impact on immune system, especially on differencial blood count and spleen index. Materials and methods: The experiment was performed on 33 Wistar rats, divided in three groups, each with 11 individuals (6 male and 5 female). Two gropus included rats treated with different dose of caffeine (40 mg/kg 100 mg/kg of body weight) while the third was control group. Treated rats were orally intubated with caffeine every 48 h, for 24 days. Afer last application blood, for differential blood analysis, was collected. Also dissection was performed, for estimation of spleen index. Results: Caffeine treated rats had highet leukocyte count. Neutrophil and eosinophil proportion was also higher in treated rats, while monocyte count declined with growth of caffeine concentration. Spllen index was higher in treated individuals. Conclusions: Higher leukocyte count shows higher immune activitey in rats treated with caffeine. That was confirmed by increase in neutofile end eosinophyle count, which represent cells that deffend organism from foreign agents. So, it can be concluded that caffeine can be treated as an agent that cause high immune activity which was correlated with increase in spleen index.

Key words:
Caffeine, Wistar rat, differential blood count, spleen

Thematic field:
Medical and health sciences

Date of abstract submission:
14.10.2014.

Conference:
7. Studenti u susret nauci sa međunarodnim učešćem, StES2014